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Complementary effect of combined bacterial-chemical pretreatment to promote enzymatic digestibility of lignocellulose biomass

Author:
Si, Mengying, Liu, Dan, Liu, Mingren, Yan, Xu, Gao, Congjie, Chai, Liyuan, Shi, Yan
Source:
Bioresource technology 2019 v.272 pp. 275-280
ISSN:
0960-8524
Subject:
Acinetobacter, Pandoraea, active sites, alkali treatment, bacteria, biomass, bioprocessing, enzymatic hydrolysis, hydrolysis, lignin, lignocellulose, rice straw, saccharification, sugars
Abstract:
Chemical pretreatment partially modified the structure of lignocellulose to enhance saccharification, leaving unaltered factors to limit further hydrolysis. To overcome these limitations, a biostrategy involving co-pretreatment combining bacteria with a chemical process was developed. A significant complementary effect was observed in specific co-pretreatments, e.g., ligninolytic bacteria enhanced acid pretreatment and saccharolytic bacteria enhanced alkaline pretreatment. Specifically, the ligninolytic bacterium Pandoraea sp. B-6 selectively removed the acidolysis-caused residual lignin and enhanced sugar release by 40.9% to 772.0 mg g−1 compared with that of acid-treated rice straw. After most of the lignin was removed, sugar release from alkali-treated RS was further improved by 31.8% to 820.2 mg g−1 via the saccharolytic bacterium Acinetobacter sp. B-2 through decrystallization. In the complementary mechanism, the active sites produced by chemical cleavage facilitated the bioprocess and further enhanced saccharification. This complementary mechanism provides a novel foundation for designing a rational combination pretreatment.
Agid:
6180497