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Identification and Transcription Profiling of Trypsin in Aedes taeniorhynchus (Diptera: Culicidae): Developmental Regulation, Blood Feeding, and Permethrin Exposure

Zhao, Liming, Chen, Jian, Becnel, James J., Kline, Daniel L., Clark, Gary G., Linthicum, Kenneth J.
Journal of medical entomology 2011 v.48 no.3 pp. 546
Aedes taeniorhynchus, Ochlerotatus, adults, amino acids, blood, complementary DNA, eggs, females, gene expression, gene expression regulation, genes, larvae, males, messenger RNA, open reading frames, permethrin, quantitative polymerase chain reaction, trypsin
The cDNA of a trypsin gene from Aedes (Ochlerotatus) taeniorhynehus (Weidemann) was cloned and sequenced. The full-length mRNA sequence (890 bp) for trypsin from Ae. taeniorhynchus (AetTryp1) was obtained, which encodes an open reading frame of 765 bp (i.e., 255 amino acids). To detect whether AetTryp is developmentally regulated, a quantitative real-time polymerase chain reaction was used to examine AetTryp1 mRNA expression levels in different developmental stages of Ae. taeniorhynehus. AetTryp1 was expressed at low levels in egg, larval, and pupal stages, but was differentially expressed in adult Ae. taeniorhynchus, with highest levels found in 5-d-old female adults when compared with teneral adults. In addition, AetTryp1 mRNA expression differed between sexes, with expression levels much lower in males. However, in both males and females, there was a significant increase in AetTryp1 transcription levels as age increased and peaked in 5-d-old adults. AetTryp1 expressed in 5-d-old female Ae. taeniorhynchus significantly increased after 30 min postblood feeding compared with the control. The AetTryp1 mRNA expression in 5-d-old female Ae. taeniorhynchus was affected by different concentrations of permethrin.