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Preparation of ferulic acid-grafted chitosan using recombinant bacterial laccase and its application in mango preservation

Yang, Jie, Sun, Jianna, An, Xiujuan, Zheng, Meixia, Lu, Zhaoxin, Lu, Fengxia, Zhang, Chong
RSC advances 2018 v.8 no.13 pp. 6759-6767
2,2-diphenyl-1-picrylhydrazyl, Bacillus subtilis, Bacillus vallismortis, Escherichia coli, Fourier transform infrared spectroscopy, Mangifera indica, Staphylococcus aureus, antibacterial properties, antioxidant activity, ascorbic acid, catalase, chitosan, coatings, ferulic acid, free radical scavengers, hydrogen peroxide, laccase, mangoes, molecular weight, peroxidase, sensory properties, titratable acidity, ultraviolet-visible spectroscopy
To improve the antioxidant and antibacterial properties of chitosan, recombinant bacterial laccase from Bacillus vallismortis fmb-103 (fmb-rL103) was used to catalyze ferulic acid grafting. The grafted chitosan was characterized using UV-vis and FT-IR techniques. DPPH free radical scavenging results indicated that the antioxidant properties of the grafted chitosan (FA-g-CS) were significantly improved. Meanwhile, the antibacterial properties against E. coli, S. aureus, B. subtilis, and M. guilliermondii were also improved. Furthermore, FA-g-CS was applied to mango preservation as a coating, which improved the sensory qualities of mango Mangifera indica L. The disease incidence of mangoes coated with FA-grafted medium and high molecular weight chitosan were 0 and 5%, respectively. The respiratory peak was delayed more than 4 days, and the titratable acidity and ascorbic acid concentration were all enhanced. POD and CAT activities in FA-g-CS coated mangoes were higher than those coated with chitosan samples with a lower H₂O₂ concentration.