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Albumin-assisted exfoliated ultrathin rhenium disulfide nanosheets as a tumor targeting and dual-stimuli-responsive drug delivery system for a combination chemo-photothermal treatment

Author:
Huang, Qunlian, Wang, Shurong, Zhou, Jie, Zhong, Xiaoyan, Huang, Yilan
Source:
RSC advances 2018 v.8 no.9 pp. 4624-4633
ISSN:
2046-2069
Subject:
absorbance, antineoplastic agents, biocompatibility, bovine serum albumin, cytotoxicity, drug delivery systems, folic acid, human cell lines, in vitro studies, in vivo studies, intravenous injection, irradiation, mice, nanocomposites, nanosheets, near-infrared spectroscopy, neoplasms, pH, relapse, resveratrol, rhenium, solubility, temperature, ultrasonics
Abstract:
Herein, we prepared an ultrathin rhenium disulfide nanosheet (utReS₂) through the bovine serum albumin (BSA)-assisted ultrasonic exfoliation method, which showed great biocompatibility and high near-infrared (NIR) absorbance. The large surface specific area and the presence of BSA facilitate a high loading ratio and modification of multifunctional molecules. The low solubility anti-cancer drug resveratrol (RSV) was loaded onto the utReS₂ surface to form a biocompatible nanocomposite (utReS₂@RSV). A targeting molecule, folic acid (FA), was then conjugated to the BSA molecule of utReS₂@RSV, resulting in utReS₂@RSV–FA. The utReS₂@RSV–FA exhibited a photothermal effect under an 808 nm laser irradiation. At pH = 6.5, about 16.5% of the RSV molecules was released from utReS₂@RSV–FA over 24 h, while the value reached 55.3% after six cycles of NIR irradiation (5 min, 1 W cm⁻²). In vitro experiments of utReS₂@RSV–FA showed that it had low cytotoxicity and an excellent HepG2 cells targeting effect. Upon pH/temperature dual-stimuli, utReS₂@RSV–FA showed an enhanced cytotoxic effect. In vivo experiments of utReS₂@RSV–FA intravenously injected into tumor-bearing mice showed that at 24 h post-injection, it could actively target and was largely accumulated in tumor tissue. When the injection was further accompanied by three cycles of NIR irradiation for 5 min, once a day, the tumor was efficiently suppressed, without relapse after 30 days. These findings demonstrate that utReS₂@RSV–FA has a remarkable targeting ability while providing a dual-stimuli-responsive drug delivery system, and could effectively be used in a combination chemo-photothermal cancer treatment.
Agid:
6182273