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Cytoprotective effects of a tripeptide from Chinese Baijiu against AAPH-induced oxidative stress in HepG2 cells via Nrf2 signaling

Author:
Wu, Jihong, Sun, Baoguo, Luo, Xuelian, Zhao, Mouming, Zheng, Fuping, Sun, Jinyuan, Li, Hehe, Sun, Xiaotao, Huang, Mingquan
Source:
RSC advances 2018 v.8 no.20 pp. 10898-10906
ISSN:
2046-2069
Subject:
antioxidant activity, antioxidants, enzymes, glutathione, health promotion, human cell lines, malondialdehyde, messenger RNA, oxidative stress, protective effect, protein synthesis
Abstract:
Antioxidant peptides have been widely reported, whereas the intracellular antioxidant activity of a tripeptide (Pro-His-Pro, PHP), which was newly isolated and identified from Chinese Baijiu in our previous study, are still poorly understood. This study investigated the protective effects of PHP on 2,2′-azobis (2-methyl-propanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells and the involved molecular mechanisms. Pretreatment with PHP suppressed the generations of reactive oxygen species (ROS), malondialdehyde (MDA) and oxidized glutathione (GSSG), prevented a decrease in reduced glutathione (GSH), and up-regulated the activities of cellular antioxidant enzymes. Moreover, PHP treatment stimulated the mRNA and protein expression levels of antioxidant enzymes and nuclear factor E2 related factor 2 (Nrf2). Meanwhile, PHP markedly reduced the level of Kelch-like ECH-associated protein 1 (Keap1), suggesting that PHP effectively activated Nrf2/antioxidant response element (ARE)-mediated activity. These findings provide the first molecular basis for the health-promoting effects of PHP to prevent AAPH-induced oxidative stress.
Agid:
6183454