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Eu/Tb luminescence for alkaline phosphatase and β-galactosidase assay in hydrogels and on paper devices
- Gorai, Tumpa, Maitra, Uday
- Journal of materials chemistry 2018 v.6 no.14 pp. 2143-2150
- alkaline phosphatase, beta-galactosidase, cholates, hydrogels, luminescence
- Simple technologies for efficient detection of important (bio)molecules are always in great demand. We now report the detection and assay of two biologically important enzymes, alkaline phosphatase and β-galactosidase, in Eu- or Tb-based cholate hydrogels, respectively, and on filter paper discs coated with such hydrogels. Pro-sensitizers derived from 1-hydroxypyrene and 2,3-dihydroxynaphthalene were incorporated into Eu or Tb cholate hydrogels, respectively. Upon enzyme action, these artificial substrates liberate free sensitizers both in the gel and on gel-coated discs, resulting in turn-on luminescence, red/magenta for Eu, and green for Tb. The detection of enzymes was also demonstrated in natural/biological samples using low-cost systems.