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Identification and transcription profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): development regulation and environmental response

Liming Zhao, Daniel L. Kline, James J. Becnel, Jian Chen, Sandra A. Allan, Gary G. Clark, Kenneth J. Linthicum
Open access insect physiology 2014 v.2015 no.5 pp. 1-12
Aedes taeniorhynchus, NAD (coenzyme), NADH dehydrogenase, Ochlerotatus, adults, amino acids, complementary DNA, double-stranded RNA, eggs, females, gene expression, genes, insect physiology, larvae, messenger RNA, mosquito control, open reading frames, permethrin, pupae, quantitative polymerase chain reaction, transcription (genetics)
The cDNA of a NADH dehydrogenase-ubiquinone Fe-S protein 8 subunit (NDUFS8) gene from Aedes (Ochlerotatus) taeniorhynchus Wiedemann has been cloned and sequenced. The 824 bp full-length mRNA sequence of AetNDUFS8 encodes an open reading region of 651 bp (that is, 217 amino acids). To identify if AetNDUFS8 is developmentally regulated, we employed a quantitative real-time polymerase chain reaction to examine AetNDUFS8 gene expression levels in all developmental stages of Ae. taeniorhynchus. In egg, larval, and pupal stages, AetNDUFS8 was expressed at relatively low levels. However, in the teneral adult stage, AetNDUFS8 was highly expressed. During the time course study in response to permethrin pesticide treatment, quantitative real-time polymerase chain reaction (PCR) also showed that mRNA transcription levels of AetNDUFS8 were regulated in female Ae. taeniorhynchus. A mitochondrially encoded NADH dehydrogenase subunit 5 AetNADH5 was highly expressed in different developmental stages of Ae. taeniorhynchus. This study suggests that AetNDUFS8 and AetNADH5 play an essential role in the development of Ae. taeniorhynchus and will provide information useful for developing dsRNA pesticide for mosquito control.