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Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae
- Deguo Wang, Yanhong Liu
- International Journal of Environmental Research and Public Health 2015 v.12 no.6 pp. 5735-5742
- DNA primers, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, bovine mastitis, dairy industry, detection limit, epidemiological studies, genes, intergenic DNA, loop-mediated isothermal amplification, microbial detection, pathogens, public health, public research, ribosomal RNA
- Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae are the three main pathogens causing bovine mastitis, with great losses to the dairy industry. Rapid and specific loop-mediated is othermal amplification methods (LAMP) for identification and differentiation of these three pathogens are not available. With the 16S rRNA gene and 16S-23S rRNA intergenic spacers as targets, four sets of LAMP primers were designed for identification and differentiation of S. dysgalactiae, S. uberis and S. agalactiae. The detection limit of all four LAMP primer sets were 0.1 pg DNA template per reaction, the LAMP method with 16S rRNA gene and 16S-23S rRNA intergenic spacers as the target s can differentiate the three pathogens, which is potentially useful in epidemiological studies.