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Combined treatment with cinnamaldehyde and PTH enhances the therapeutic effect on glucocorticoid-induced osteoporosis through inhibiting osteoclastogenesis and promoting osteoblastogenesis

Wu, Zongyi, Yan, Deyi, Xie, Zhongjie, Weng, Sheji, Zhou, Qiang, Li, Hang, Bai, Bingli, Boodhun, Viraj, Shen, Zijian, Tang, Jiahao, Yang, Lei
Biochemical and biophysical research communications 2018 v.505 no.3 pp. 945-950
acid phosphatase, alkaline phosphatase, bone formation, dexamethasone, eosin, femur, gene expression regulation, immunohistochemistry, in vitro studies, intragastric administration, laboratory animals, males, mesenchymal stromal cells, mice, micro-computed tomography, osteoporosis, parathyroid hormone, rats, staining, subcutaneous injection, therapeutics, transcription factors
The study was to investigate the effect of combining treatment with cinnamaldehyde and parathyroid hormone (1−34) (PTH) on glucocorticoid-induced osteoporosis (GIO) and compare with monotherapy. Forty Sprague-Dawley male rats with GIO were divided into four groups randomly: control group (CON group, N = 10); group that intragastric administration with cinnamaldehyde (CIN group, N = 10); group that subcutaneous injection with PTH, three times per week(PTH group, N = 10); both administration with cinnamaldehyde and PTH (CIN + PTH group, N = 10). Distal femurs were harvested for hematoxylin and eosin (H&E) staining, micro-CT scanning and immunohistochemical analysis. Murine mesenchymal stem cells were cultured and dealt with the presence of dexamethasone(DEX group), DEX + cinnamaldehyde(DEX + CIN group), DEX + PTH(DEX + PTH group) or DEX + cinnamaldehyde + PTH(DEX + CIN + PTH group). Alkaline phosphatase (ALP) staining was performed subsequently. The results showed that bone formation in CIN + PTH group was notably promoted compared with other groups. And the expression of tartrate-resistant acid phosphatase (trap) and runt-related transcription factor 2 (runx2) in CIN + PTH group were down-regulated and up-regulated respectively compared with PTH group. In vitro study revealed that ALP-positive cell number in DEX + CIN + PTH group was obviously enhanced compared with other groups. The study revealed that combined treatment with cinnamaldehyde and PTH enhances the therapeutic effect on GIO through inhibiting osteoclastogenesis and promoting osteoblastogenesis.