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Preparation of a stable and robust nanobiocatalyst by efficiently immobilizing of pectinase onto cyanuric chloride-functionalized chitosan grafted magnetic nanoparticles

Soozanipour, Asieh, Taheri-Kafrani, Asghar, Barkhori, Masoumeh, Nasrollahzadeh, Mahmoud
Journal of colloid and interface science 2019 v.536 pp. 261-270
catalytic activity, chemical bonding, chitosan, circular dichroism spectroscopy, cyanuric chloride, encapsulation, immobilized enzymes, magnetism, magnetite, nanoparticles, pH, polygalacturonase, storage quality, storage temperature
In industrial processes, effective degradation of polygalacturonic acid using immobilized pectinase is preferred over free one due to its stability and efficient functional reuses. Pectinase was covalently conjugated to the surface of cyanuric chloride functionalized chitosan encapsulated magnetite nanoparticles. The results obtained of various analytical tools and biochemical studies demonstrated successful synthesis and immobilization processes, high immobilization efficiency and loading capacity. The circular dichroism (CD) results of free and immobilized pectinase revealed the partial decreases in the α-helices and β-sheets, and marginal increases in the unordered elements contents of pectinase upon the immobilization onto Fe3O4@Ch-CC nanoparticles, along with stability improvement. The immobilized pectinase was retained about 60% of its initial catalytic activity after 13 recycles at optimum conditions (40 °C, pH 4.5). The storage stability of pectinase was increased due to immobilization, after 75 days storage at 4 °C, the free and immobilized enzyme retained 43% and 74% of the initial activity, respectively. The immobilized pectinase showed higher storage stability and better performance at wider ranges of pH and temperature, compared to free pectinase.