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Extraction, partial purification and characterization of alkaline protease from rainbow trout (Oncorhynchus Mykiss) viscera

Taghizadeh Andevari, Ghasem, Rezaei, Masoud, Tabarsa, Mehdi, Rustad, Turid
Aquaculture 2019 v.500 pp. 458-463
Oncorhynchus mykiss, ammonium sulfate, animal organs, collagenase, detergents, digestive tract, enzyme activity, industry, molecular weight, pH, serine proteinases, solvents, soybeans, surfactants, temperature, thermal stability, trypsin inhibitors
In this study, crude alkaline proteases were recovered from rainbow trout (Oncorhynchus mykiss) viscera and partially purified by use of different saturation of ammonium sulfate. The enzyme exhibited highest yield, purity and activity when precipitated at a saturation of 40–60% compared to other ranges of saturation. Molecular weight for extracted protease was between 8-24 kDa. The protease had caseinolytic activity over a wide range of temperatures (30-55 °C) and pH (4-12). Soybean trypsin inhibitor and trypsin-chymotrypsin inhibitor strongly inhibited the enzyme activity but it was stable in the presence of surfactant, oxidizing reagents and organic solvents. The proteases had serine protease activity but no collagenase activity was detected. The current study showed that partially purified protease from the digestive tract of rainbow trout could be applicable in food and detergent industry because of its good activity over a wide temperature and pH range and its good thermal stability.