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Rhizobacterium-mediated growth promotion and expression of stress enzymes in Glycine max L. Merrill against Fusarium wilt upon challenge inoculation

Author:
Jain, Shekhar, Vaishnav, Anukool, Kasotia, Amrita, Kumari, Sarita, Gaur, Rajarshi Kumar, Choudhary, Devendra Kumar
Source:
World journal of microbiology & biotechnology 2014 v.30 no.2 pp. 399-406
ISSN:
0959-3993
Subject:
1-aminocyclopropane-1-carboxylate deaminase, Carnobacterium, Fusarium oxysporum, Fusarium wilt, Glycine max, Rhizobium, bacteria, chlorophyll, fungi, growth promotion, induced resistance, leaves, pathogens, polymerase chain reaction, salt stress, seeds, sowing, soybeans
Abstract:
Wilt disease of soybean caused by a very common soil-borne fungus, Fusarium oxysporum is one of the most destructive diseases of the crop. The aim of the present study was to characterize plant growth-promotion activities and induced resistance of a rhizobacterial strain for the soybean plant against F. oxysporum. Rhizobacterium strain SJ-5 exhibited plant growth-promotion characteristics and antagonistic activity against the test pathogen on dual plate assay. It was identified as a Carnobacterium sp. A 950 bp PCR product was amplified from Carnobacterium sp. strain SJ-5, using zwittermicin A self-resistance gene-specific primers (zmaR). The strain produced indole 3-acetic acid (19 μg/ml) in the presence of salt stress and exhibited growth in Dworkin and Foster salt medium amended with 1-aminocyclopropane-1-carboxylate (ACC) through ACC deaminase activity (277 nmol/mg/h) as compared to the control. Strain seeds treated with the strain significantly enhanced the quorum of healthy plants after challenge inoculation at 14 days after seeding. An increase in the activity of stress enzymes after challenge inoculation with the test pathogen is reported. Treatment with the bacterium resulted in an increase in the chlorophyll content in the leaves in comparison with challenge-inoculated plants.
Agid:
619130