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Derivation of chicken primordial germ cells using an indirect Co-culture system
- Xie, Long, Lu, Zhenping, Chen, Dongyang, Yang, Mengmeng, Liao, Yuying, Mao, Wenrong, Mo, Lifen, Sun, Juanjuan, Yang, Wenhao, Xu, Huiyan, Lu, Kehuan, Lu, Yangqing
- Theriogenology 2019 v.123 pp. 83-89
- biotechnology, chick embryos, chickens, coculture, genomics, germ cells, gonads, phenotype, progeny
- Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing.