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Hemostasis and anti-necrotic activity of wound-healing dressing containing chitosan nanoparticles

Biranje, Santosh S., Madiwale, Pallavi V., Patankar, Kaustubh C., Chhabra, Rohan, Dandekar-Jain, Prajakta, Adivarekar, Ravindra V.
International journal of biological macromolecules 2019 v.121 pp. 936-946
biodegradability, biodegradation, blood, blood coagulation, chitosan, enzyme-linked immunosorbent assay, fibroblasts, freeze drying, gelation, hemostasis, humans, nanoparticles, porosity, surface area, thrombin, tissue repair
Necrotic tissues are the dead tissues present in the wounded areas, which need to be removed for rapid wound healing. Various biopolymer-based dressings have been exploited to heal infected wounds, but with limited success. In a quest to develop an effective and economic wound dressing, a biodegradable dressing containing chitosan nanoparticles has been successfully developed. Chitosan nanoparticles were prepared by ionic gelation method and then assembled into the porous chitosan dressing, by lyophilization. The resulting dressing was analyzed for morphology, porosity, pore volume, surface area and biodegradability. Higher surface area and porosity of the dressing facilitated its partial biodegradation by enzymatic action. In vitro cellular investigations with Human Dermal Fibroblasts (HDF) confirmed the safety of the dressing for wound healing applications. Human Thrombin-Antithrombin (TAT) based in vitro ELISA assay, for evaluating the hemostasis activity, illustrated an accelerated hemostasis activity, through higher thrombin generation and stable blood clot formation. The blood in contact with the dressing contained two-fold higher levels of TAT, as compared to that in contact with the TAT standard. Our results suggest the potential of the developed dressing for removing the necrotic tissues and accelerating the hemostasis activity, for efficient and rapid wound healing.