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Analysis of differentially expressed proteins in the pollen of Malus spectabilis (crabapples) and Malus domestica (‘Gala’ apples) and their role in pollination

Wang, Rong, Shen, Xiang, Zhang, Wenhui
Journal of horticultural science & biotechnology 2018 v.93 no.6 pp. 566-572
Malus domestica, actin, apples, beta-fructofuranosidase, chloroplasts, cytochrome b, databases, ferritin, phosphoglucomutase, pollen, pollination, protein subunits, protein synthesis, proton pump, regulatory proteins, signal transduction, tandem mass spectrometry, two-dimensional gel electrophoresis
Our objective was to study the differentially expressed proteins (DEP) in various Malus spectabilis (crabapple) varieties (M. ‘Snowdrift’, M. ‘Hongling’ and M. ‘Hongjin’) in relation to Malus domestica (‘Gala’) and their role in pollination. Our method used a two-dimensional electrophoresis (2-DE) to analyse the differential proteins in the pollen of several crabapples. The 2-DE apples combined with the tandem mass spectrometry (MS-MS) and protein database retrieval helped us to identify the nature and function of DEPs in ‘Gala’ apples and crabapples. We identified 1195 proteins through 2-DE. Among these, six DEPs, namely chloroplast ferritin, Actin, Beta-fructofuranosidase, vacuolarH+-ATPase catalytic subunit, Full = Phosphoglucomutase, and Cytochrome b were identified by MS-MS. This study identified six DEPs among the pollen from the ‘Snowdrift’ crabapple, ‘Hongling’ crabapple, ‘Hongjin’ crabapple, and ‘Gala’ apples. The DEPs included metabolism related proteins, stress/regulatory proteins, and proteins involved in signal transduction.