Main content area

Direct and fast capture lactoferrin from cheese whey on nanoparticles of Fe3O4 combined with concanavalin A

Zhang, J.L., Di, W., Gong, P.M., Lin, K., Lyu, L.Z., Zhang, L.W., Han, X., Ma, Y.
Food chemistry 2019 v.274 pp. 314-318
adsorption, cheese whey, concanavalin A, dose response, iron oxides, lactoferrin, magnetism, nanoparticles, phosphates, scanning electron microscopes, whey
Ferric and ferrous were used to prepare the magnetic nanoparticles (MNPs), and concanavalin A was bound onto the MNPs as a magnetic nano-adsorbent for lactoferrin (Lf) separation. Scanning electron microscope showed that the diameter of modified MNPs was about 15.27 ± 1.42 nm. The results showed that the optimum adsorption and elution conditions of modified MNPs on recovery Lf were 4 min and 5 min, respectively. The specificity of modified MNPs on recovery Lf was high, and the purity of Lf in eluent was 93.06%. The recovery rate of modified MNPs from whey and elution were more than 99.99%, and the recovery rate of Lf from whey performed a dose-dependent relationship. The maximum adsorption capacity of modified MNPs on recovery Lf was 90 µg/mg. The adsorption capacity of modified MNPs stored in phosphate buffer at 4 °C significantly higher (P < 0.05) than those stored in other experimental conditions.