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A colorimetric paper-based sensor for toltrazuril and its metabolites in feed, chicken, and egg samples
- Wang, Zhongxing, Zhang, Jian, Liu, Liqiang, Wu, Xiaoling, Kuang, Hua, Xu, Chuanlai, Xu, Liguang
- Food chemistry 2019 v.276 pp. 707-713
- European Union, chickens, colorimetry, detection limit, eggs, haptens, inhibitory concentration 50, metabolites, monoclonal antibodies, rapid methods, screening, toltrazuril
- In this study, a sensitive monoclonal antibody (mAb) against toltrazuril (Tol) was developed based on a novel hapten. The 50% inhibitory concentrations (IC50) of toltrazuril and its metabolites ranged from 2.19 ng/mL to 4.21 ng/mL. Based on this mAb, a colorimetric paper-based sensor was developed for the rapid screening of Tol and its metabolites in samples. The proposed assay has cutoff values of <20 μg/kg for Tol and 50 μg/kg for Tol sulfone when evaluated with the naked eye, and the results could be obtained in 15 min. Quantitative results were obtained with a strip scan reader, with limits of detection <2.60 μg/kg for Tol and its metabolites in real samples. The sensitivity of both qualitative and quantitative detection meets the European Union requirements. Therefore, this strip assay provides a useful tool for the on-site detection and rapid initial screening of Tol and its derivatives in feed, egg, and chicken samples.