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Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin–Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay

Author:
Jiang, Wenxiao, Beier, Ross C., Luo, Pengjie, Zhai, Peng, Wu, Nan, Lin, Guimiao, Wang, Xiaomei, Xu, Gaixia
Source:
Journal of agricultural and food chemistry 2016 v.64 no.1 pp. 364-370
ISSN:
0021-8561
Subject:
antibiotic residues, public health, drug residues, antibody detection, beef, detection limit, enzyme-linked immunosorbent assay, food contamination, honey, inhibitory concentration 50, milk, monitoring, monoclonal antibodies, muscles, pirlimycin, screening, veterinary drugs
Abstract:
Food contamination by veterinary drug residues is a worldwide public health concern and requires continuous monitoring. In this study, we developed a biotin–streptavidin-amplified ELISA (BA-ELISA) using a produced monoclonal antibody for detecting pirlimycin residues in beef muscle, milk, and honey. The IC50 value of the BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity was improved 3 times compared to traditional ELISAs. The optimized BA-ELISA can be used to quantitate trace amounts of pirlimycin residues in beef muscle, milk, and honey. This method had limits of detection (LODs) of 4.45 μg/kg in beef muscle, 1.65 μg/L in milk, and 2.75 μg/kg in honey. The average recovery of the BA-ELISA ranged from 78 to 97%, and the coefficient of variation ranged from 5.3 to 13.5%. The developed BA-ELISA method was validated using LC-MS/MS, and the BA-ELISA can be used for routine screening analysis of pirlimycin residues.
Agid:
61964
Handle:
10113/61964