Main content area

Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin–Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay

Jiang, Wenxiao, Beier, Ross C., Luo, Pengjie, Zhai, Peng, Wu, Nan, Lin, Guimiao, Wang, Xiaomei, Xu, Gaixia
Journal of agricultural and food chemistry 2016 v.64 no.1 pp. 364-370
antibiotic residues, public health, drug residues, antibody detection, beef, detection limit, enzyme-linked immunosorbent assay, food contamination, honey, inhibitory concentration 50, milk, monitoring, monoclonal antibodies, muscles, pirlimycin, screening, veterinary drugs
Food contamination by veterinary drug residues is a worldwide public health concern and requires continuous monitoring. In this study, we developed a biotin–streptavidin-amplified ELISA (BA-ELISA) using a produced monoclonal antibody for detecting pirlimycin residues in beef muscle, milk, and honey. The IC50 value of the BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity was improved 3 times compared to traditional ELISAs. The optimized BA-ELISA can be used to quantitate trace amounts of pirlimycin residues in beef muscle, milk, and honey. This method had limits of detection (LODs) of 4.45 μg/kg in beef muscle, 1.65 μg/L in milk, and 2.75 μg/kg in honey. The average recovery of the BA-ELISA ranged from 78 to 97%, and the coefficient of variation ranged from 5.3 to 13.5%. The developed BA-ELISA method was validated using LC-MS/MS, and the BA-ELISA can be used for routine screening analysis of pirlimycin residues.