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Protein analysis of moro blood orange pulp during storage at low temperatures

Carmona, L., Alquézar, B., Tárraga, S., Peña, L.
Food chemistry 2019 v.277 pp. 75-83
Citrus sinensis, amino acids, anthocyanins, biogenesis, biosynthesis, chalcone, fruits, glutathione transferase, orange pulp, oranges, oxidative stress, storage temperature, storage time, stress response, structural proteins, sugars, vacuoles
A protein analysis in the pulp of Moro blood oranges (Citrus sinensis L. Osbeck) at the onset and after 30 days of storage at either 4 or 9 °C was performed. All differential proteins belonged to different functional classes (sugar, amino acid and secondary metabolism, defense, stress response, oxidative process, transport and cellular component biogenesis), displaying a differential accumulation in those Moro oranges kept at 9 versus 4 °C, and in those stored at 4 °C versus onset. Anthocyanin biosynthesis structural proteins chalcone synthases and flavonone 3-hydroxylase and different glutathione S-transferases related with their vacuolar transport were up-accumulated in fruits kept at 9 versus 4 °C and versus the onset. Proteins related with defense and oxidative stress displayed a similar pattern, concomitant with a higher anthocyanin content, denoting a possible role of defense and other stress response pathways in anthocyanin production/accumulation.