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Point-of-care-testing of α-amylase activity in human blood serum

Author:
Mandal, Nilanjan, Bhattacharjee, Mitradip, Chattopadhyay, Arun, Bandyopadhyay, Dipankar
Source:
Biosensors & bioelectronics 2019 v.124-125 pp. 75-81
ISSN:
0956-5663
Subject:
alpha-amylase, biosensors, blood serum, chemical reactions, digestion, electrical resistance, enzyme activity, glass, humans, ions, nanogold, neoplasms, pancreatitis, starch
Abstract:
Activity of α-amylase enzyme in human serum indicates the onset of pancreatitis, mumps, cancer, stress, and depression. Herein we design and develop a biosensor for the point-of-care-testing (POCT) of α-amylase concentration in serum. The biosensor is composed of a glass substrate coated with an electrically conducting poly-aniline-emeraldine-salt (PANI-ES) film covered with starch-coated gold nanoparticles (SAuNPs). Addition of different dosage of α-amylase on the biosensor selectively depletes starch stabilized on the SAuNPs, which changes the electrical resistance of the sensor. The change in electrical resistance show a nearly linear correlation with the concentration of α-amylase in buffer, which helps the detection of unknown α-amylase activity in the blood serum. The biosensor responds in a specific manner owing to the use of selective enzymatic chemical reaction between α-amylase and starch. The pathways to SAuNP formation on PANI-ES, time-dependent starch digestion with α-amylase, and the subsequent variation in electrical response was characterized to uncover the sensing mechanism. The chloride ions and the AuNPs present catalyse the starch-amylase reaction on the PANI surface to enable a sensitive detection of α-amylase in serum (25 – 100 U/l) at a quick response time of ~60 s. Integration of the biosensor with the built-in sourcemeter and a real time display help an immediate presentation of α-amylase level in the serum, comparable to the clinically approved methodologies.
Agid:
6197150