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Heart and liver regeneration in zebrafish using silver nanoparticle synthesized from Turbinaria conoides – In vivo

Ali, M. Syed, Anuradha, V., Yogananth, N., Krishnakumar, S.
Biocatalysis and agricultural biotechnology 2019 v.17 pp. 104-109
Danio rerio, Fourier transform infrared spectroscopy, Turbinaria conoides, X-ray diffraction, bioactive properties, cardiomyocytes, heart rate, hydrogen peroxide, hypertrophy, liver, liver regeneration, models, nanoparticles, nanosilver, plant extracts, scanning electron microscopy, silver nitrate, staining, vertebrates, viability
The present study was aimed to identify the heart and liver regeneration in zebrafish using biosynthesised silver nanoparticles from Turbinaria conoides. This work focussed on the bioactivity of AgNP when incorporated into the zebrafish (Danio rerio) system. We began investigating the in vivo assays: Hepatocyte viability staining after H2O2 treatment, cardiomyocyte response to Ca++, cardio vascular heart rate activity by measuring hypertrophy, cardio vascular pathology and cardio vascular regeneration, liver regeneration and liver pathology, molecular pathway target identification and hypothesis on interacting domain (AgNO3) of the vertebrate model organism. The FTIR results of biosynthesized silver nanoparticles from Turbinaria conoides showed prominent peaks (range between 620.967 and 2854.14). Further, the results of XRD analysis showed the 2γ intense values (38.11 and 70.57) within the ranges of Bragg's reflection. In addition, the SEM analysis determined the size of nanoparticle (50–100 nm). It can be concluded from the present findings that, the biosynthesised silver nanoparticles from the seaweed extract of Turbinaria conoides can be used as potential cardioprotective and liver protective agent.