U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

De novo construction of an expanded transcriptome assembly for the western tarnished plant bug, Lygus hesperus

Erica E. Tassone, Scott M. Geib, Brian Hall, Jeffrey A. Fabrick, Colin S. Brent, J. Joe Hull
GigaScience 2016 v.5 no.6 pp. 1-5
Lygus hesperus, crops, data collection, genes, insect pests, insect physiology, open reading frames, peptides, phytophagous insects, plant pests, proteins, tissue distribution, transcriptome
The plant bug, Lygus hesperus Knight, is a polyphagous pest of many economically important crops. Despite its pest status, little is known about the molecular mechanisms responsible for much of Lygus biology. Earlier Lygus transcriptome assemblies were either limited by low read depth or focused on specific conditions. To generate a more comprehensive transcriptome, we have supplemented previous datasets with further reads corresponding to specific tissues (heads, antennae, and male reproductive tissues). This expanded transcriptome offers greater insight into the molecular genetics of Lygus, and provides greater foundational knowledge from which comparative studies with other plant bugs can be made. Findings: A de novo transcriptome assembly was generated from multiple libraries comprising different tissues and thermal conditions. The transcriptome consists of 22,022 transcripts (average length of 2075 nt), 62% of which contain complete open reading frames. Transcriptome metrics are consistent with a robust assembly, with both CEGMA and BUSCO analyses showing a high degree of completeness. In addition, more transcripts with homology to genes encoding proteins/peptides critical to regulating insect physiology and chemosensation were identified in the current assembly than previous iterations. Conclusions: This transcriptome represents a significant expansion of Lygus transcriptome data and improves foundational knowledge about the molecular mechanisms underlying L. hesperus biology. The dataset is publically available in NCBI and GigaDB as a resource for researchers.