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Induced androgenesis as a biotechnology method for obtaining DH plants in Daucus carota L.

Kiszczak, W., Kowalska, U., Burian, M., Gorecka, K.
Journal of horticultural science & biotechnology 2018 v.93 no.6 pp. 625-633
2,4-D, Daucus carota, acclimation, amino acids, androgenesis, anthers, aspartate transaminase, biotechnology, chromosomes, doubled haploids, glucose-6-phosphate isomerase, growth chambers, homozygosity, hormones, horticulture, isozymes, naphthaleneacetic acid, plant adaptation, shoots, tetraploidy, umbels
The influence of various factors on the efficiency of obtaining double haploid lines in anther cultures was examined in Daucus carota L. The impact of the genotype, the formation of donor plants and the growth conditions was investigated. The effects of various regeneration media were analysed. Acclimatisation of androgenic plants and their evaluation were also conducted. Androgenesis was induced on B5 medium containing 2,4-D and NAA at 0.1 g·L−1. Depending on the genotype, 1.2–305.3 embryos per 100 anthers were obtained. The highest number of embryos (5.5 per 100 anthers) was obtained from donor plants with the first-order shoots containing one umbel. Cultivation of donor plants under controlled conditions improved the number of embryos from 305 in the open field to 1764 in the growth chamber. Regeneration of plants from androgenetic embryos proceeded most effectively on B5 medium without hormones and amino acids. Regenerated plants adapted in over 60%. Ploidy analysis showed the presence of 92% of plants with a doubled chromosome set and 8% with a tetraploid number of chromosomes. Plants with a doubled chromosome set were 73% homozygous for the glucose phosphate isomerase isoenzyme and 100% homozygous for the aspartate aminotransferase isoenzyme, which confirms their gametic origin.