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Drought Stress Effects on Growth, ROS Markers, Compatible Solutes, Phenolics, Flavonoids, and Antioxidant Activity in Amaranthus tricolor

Sarker, Umakanta, Oba, Shinya
Applied biochemistry and biotechnology 2018 v.186 no.4 pp. 999-1016
Amaranthus tricolor, antioxidant activity, ascorbic acid, biomass, breeding programs, carotenoids, chlorophyll, cultivars, drought, field capacity, flavonoids, hydrogen peroxide, irrigation management, leaf area, new variety, photosynthesis, polyphenols, proline, solutes, water content, water stress
Four selected Amaranthus tricolor cultivars were grown under four irrigation regimes (25, 50, 80, and 100% field capacity) to evaluate the mechanisms of growth and physiological and biochemical responses against drought stress in randomized complete block design with three replications. Drought stress led to decrease in total biomass, specific leaf area, relative water content (RWC), photosynthetic pigments (chlorophyll a, chlorophyll b, chlorophyll ab), and soluble protein and increase in MDA, H₂O₂, EL, proline, total carotenoid, ascorbic acid, polyphenols, flavonoids, and antioxidant activity. However, responses of these parameters were differential in respect to cultivars and the degree of drought stresses. No significant difference was observed in control and LDS for most of the traits. The cultivars VA14 and VA16 were identified as more tolerant to drought and could be used for further evaluations in future breeding programs and new cultivar release programs. Positively significant correlations among MDA, H₂O₂, compatible solutes, and non-enzymatic antioxidant (proline, TPC, TFC, and TAC) suggested that compatible solutes and non-enzymatic antioxidant played vital role in detoxifying of ROS in A. tricolor cultivar. The increased content of ascorbic acid indicated the crucial role of the ASC–GSH cycle for scavenging ROS in A. tricolor.