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Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection

Author:
Muenchen, Daniela Kunkel, Martinazzo, Janine, Brezolin, Alexandra Nava, de Cezaro, Alana Marie, Rigo, Aline Andressa, Mezarroba, Mateus Nava, Manzoli, Alexandra, de Lima Leite, Fábio, Steffens, Juliana, Steffens, Clarice
Source:
Applied biochemistry and biotechnology 2018 v.186 no.4 pp. 1061-1073
ISSN:
0273-2289
Subject:
Cucurbita pepo, adsorption, atomic force microscopy, enzyme activity, glyphosate, peroxidase, surface tension, zucchini
Abstract:
A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg L⁻¹) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg L⁻¹.
Agid:
6199623