PubAg

Main content area

DIA+: A Data-Independent Acquisition Method Combining Multiple Precursor Charges to Improve Peptide Signal

Author:
Borràs, Eva, Sabidó, Eduard
Source:
Analytical chemistry 2018 v.90 no.21 pp. 12339-12341
ISSN:
1520-6882
Subject:
ions, peptides, proteome, proteomics
Abstract:
Data-independent acquisition methods that acquire fragment ions from virtually any peptide in a sample have expanded the benefits of low-throughput targeted proteomics to proteome-wide analyses. While these methods have increased the reproducibility of peptide quantification across multiple samples, their sensitivity is still limited, and the quantification of complete proteomes remains a challenge. Here we present DIA+, a DIA method that combines signals from identical peptides with different charge states, resulting in improved signal-to-noise, additional number of fragments, and therefore in a higher number of identified and quantified peptides in complex samples.
Agid:
6201599