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Bioconversion of Beetle-killed Lodgepole Pine Using SPORL: Process Scale-up Design, Lignin Coproduct, and High Solids Fermentaton Without Detoxification

Author:
Zhou, Haifeng, Zhu, J. Y., Luo, Xiaolin, Leu, Shao-Yuan, Wu, Xiaolei, Gleisner, Roland, Dien, Bruce S., Hector, Ronald E., Yang, Dongjie, Qiu, Xueqing, Horn, Eric, Negron, Jose
Source:
Industrial and engineering chemistry research 2013 v.52 no.45 pp. 16057
ISSN:
0888-5885
Subject:
Dendroctonus ponderosae, Pinus contorta var. latifolia, Saccharomyces cerevisiae, biomass, biotransformation, coproducts, dispersibility, endo-1,4-beta-glucanase, engineering, ethanol, fermentation, hydrolysis, lignin, lignosulfonates, pH, reaction kinetics, saccharification, slurries, sodium bisulfite, softwood, temperature, total solids, wood chips
Abstract:
Mountain pine beetle killed Lodgepole pine (Pinus contorta Douglas ex Loudon) wood chips were pretreated using an acidic sulfite solution of approximately pH = 2.0 at a liquor to wood ratio of 3 and sodium bisulfite loading of 8 wt % on wood. The combined hydrolysis factor (CHF), formulated from reaction kinetics, was used to design a scale-up pretreatment on 2000 g wood chips at a relatively low temperature of 165 °C that reduced furan formation and facilitated high solids saccharification and fermentation. The pretreated solids and liquor were disk milled together to result in a biomass whole slurry of 25% total solids. The whole biomass slurry was directly used to conduct simultaneous enzymatic saccharification and combined fermentation (SSCombF) using a commercial cellulase and Saccharomyces cerevisiae YRH400 without detoxification. A terminal ethanol titer of 47.1 g L(−1) with a yield of 306 L (tonne wood)(−1), or 72.0% theoretical, was achieved when SSCombF was conducted at an unwashed solids loading of 18%. The lignosulfonate (LS) from SPORL was highly sulfonated and showed better dispersibility than a high purity commercial softwood LS, and therefore has potential as a directly marketable coproduct.
Agid:
62065
Handle:
10113/62065