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Disposable amperometric immunosensor for Saccharomyces cerevisiae based on carboxylated graphene oxide-modified electrodes

Author:
Borisova, Boryana, Sánchez, Alfredo, Soto-Rodríguez, Paul E. D., Boujakhrout, Abderrahmane, Arévalo-Villena, María, Pingarrón, José M., Briones-Pérez, Ana, Parrado, Concepción, Villalonga, Reynaldo
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.30 pp. 7901-7907
ISSN:
1618-2642
Subject:
Saccharomyces cerevisiae, carboxylation, electrodes, enzymes, graphene, graphene oxide, immunosensors, polyclonal antibodies, storage quality, water solubility, white wines, yeasts
Abstract:
A sensitive and disposable amperometric immunosensor for Saccharomyces cerevisiae was constructed by using carbon screen-printed electrodes modified with propionic acid-functionalized graphene oxide as transduction element. The affinity-based biosensing interface was assembled by covalent immobilization of a specific polyclonal antibody on the carboxylate-enriched electrode surface via a water-soluble carbodiimide/N-hydroxysuccinimide coupling approach. A concanavalin A-peroxidase conjugate was further used as signaling element. The immunosensor allowed the amperometric detection of the yeast in buffer solution and white wine samples in the range of 10–10⁷ CFU/mL. This electroanalytical device also exhibited low detection limit and high selectivity, reproducibility, and storage stability. The immunosensor was successfully validated in spiked white wine samples.