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QTL-seq and marker development for resistance to Fusarium oxysporum f. sp. niveum race 1 in cultivated watermelon
- Branham, Sandra E., Patrick Wechter, W., Lambel, Shaunese, Massey, Laura, Ma, Michelle, Fauve, Julie, Farnham, Mark W., Levi, Amnon
- Molecular breeding 2018 v.38 no.11 pp. 139
- Fusarium oxysporum f. sp. niveum, Fusarium wilt, alleles, breeding programs, cultivars, fungi, genotyping, germplasm, polymerase chain reaction, quantitative trait loci, sequence analysis, watermelons
- Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. niveum (Fon), is one of the predominant diseases of watermelon. Resistance to Fon race 1 is conferred by a single major quantitative trait locus (QTL), Fo-1.1, but resolution of this region has been poor due to low marker density. In this study, a combination of whole genome resequencing of bulked segregants (QTL-seq analysis) followed by QTL mapping with kompetitive allele specific PCR (KASP) markers developed across Fo-1.1 successfully increased the resolution from 2.03 to 1.56 Mb and 315 kb, respectively. The linkage of the KASP markers to Fon race 1 resistance across a wide range of watermelon germplasm was validated in a set of elite watermelon cultivars. The linked markers described here provide a breeder-friendly toolkit immediately available for high-throughput genotyping in large-scale breeding programs for fine mapping and incorporation of Fon race 1 resistance in watermelon.