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LC–ESI–MS based characterisation of isoflavones in soybean (Glycine max (L.) Merr.) from India

Akitha Devi, M. K., Sravan Kumar, S., Giridhar, P.
Journal of food science and technology 2018 v.55 no.12 pp. 5045-5054
Glycine max, beans, daidzein, developmental stages, electrospray ionization mass spectrometry, genistein, glucosides, glycitein, high performance liquid chromatography, seed development, soybeans, India
A total of twenty-one soybean varieties were screened for their morphological characteristics followed by isoflavone content analysis by HPLC. The total isoflavone (TI) content was found within a wide range of 140.9–1048.6 μg/g of soy in different varieties. The highest isoflavone content was found in MAUS-2 followed by DS_2613 and lowest in Karunae (140.9 μg/g of soy). Various isoflavone forms were identified by LC–ESI⁺ MS. Significant differences in the isoflavone content were observed for all the aglycones and their glucoside conjugates as well as total daidzein, total genistein, total glycitein, and TI. A positive correlation between TI content and growth stages was found during the progression of seed development. An increase of 5.4-fold and 5.3-fold of TI concentration was observed for JS 335 and MAUS-2 respectively, from early to green mature (R5–R8) stage of bean development.