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LC–ESI–MS based characterisation of isoflavones in soybean (Glycine max (L.) Merr.) from India

Author:
Akitha Devi, M. K., Sravan Kumar, S., Giridhar, P.
Source:
Journal of food science and technology 2018 v.55 no.12 pp. 5045-5054
ISSN:
0022-1155
Subject:
Glycine max, beans, daidzein, developmental stages, electrospray ionization mass spectrometry, genistein, glucosides, glycitein, high performance liquid chromatography, seed development, soybeans, India
Abstract:
A total of twenty-one soybean varieties were screened for their morphological characteristics followed by isoflavone content analysis by HPLC. The total isoflavone (TI) content was found within a wide range of 140.9–1048.6 μg/g of soy in different varieties. The highest isoflavone content was found in MAUS-2 followed by DS_2613 and lowest in Karunae (140.9 μg/g of soy). Various isoflavone forms were identified by LC–ESI⁺ MS. Significant differences in the isoflavone content were observed for all the aglycones and their glucoside conjugates as well as total daidzein, total genistein, total glycitein, and TI. A positive correlation between TI content and growth stages was found during the progression of seed development. An increase of 5.4-fold and 5.3-fold of TI concentration was observed for JS 335 and MAUS-2 respectively, from early to green mature (R5–R8) stage of bean development.
Agid:
6207842