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Near-infrared spectroscopic evaluation of single-kernel deoxynivalenol accumulation and Fusarium head blight resistance components in wheat

Kamaranga H.S. Peiris, William W. Bockus, Floyd E. Dowell
Cereal chemistry 2016 v.93 no.1 pp. 25-31
Fusarium, Fusarium head blight, Triticum aestivum, cultivars, deoxynivalenol, disease resistance, inflorescences, near-infrared spectroscopy, pathogens, seeds, wheat
Fusarium head blight (FHB) symptoms, single kernel deoxynivalenol (DON) levels, and distribution of DON levels among kernels were investigated in wheat cultivars that had different levels of FHB resistance. DON levels were estimated using near-infrared spectroscopy (NIRS). The percentage of DON-containing spikelets/spike (DCS) of 15.2%, 49.7% and 89.1% were significantly different among point inoculated spikes of Everest that has moderate resistance, Karl 92 that has intermediate resistance, and Overley that is susceptible to FHB, respectively. The percentage of visually Fusarium damaged kernels (VFDK) in point inoculated Karl 92 and Overley spikes was significantly higher than for Everest. However, DCS and VFDK values for spray inoculated spikes were not significantly different among the three cultivars. In spray inoculated spikes, DON levels in kernels ranged from 0 - 291.3 ppm while the variation of DON levels in spikelet positions was random. In contrast, DON levels in spikelets below the inoculated spikelet in point inoculated spikes showed marked differences among the three cultivars. Overley had the highest DON accumulation in kernels. This NIRS method may be used to evaluate wheat cultivars for FHB resistance components such as resistance to pathogen infection, resistance to pathogen spread, and resistance to toxin accumulation.