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Nocardia bhagyanesis sp. nov., a novel actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis), India

Author:
Vaddavalli, Radha, Peddi, Sneha, Kothagauni, Srilekha Yadav, Linga, Venkateswar Rao
Source:
Antonie van Leeuwenhoek 2014 v.105 no.3 pp. 443-450
ISSN:
0003-6072
Subject:
Callistemon citrinus, DNA, Nocardia, arabinose, cell walls, fatty acids, nucleotide sequences, peptidoglycans, phylogeny, rhizosphere, ribosomal RNA, sequence homology, India
Abstract:
A novel actinomycete strain, designated VRC07ᵀ, was isolated from a Callistemon citrinus rhizosphere sample collected from Hyderabad, India. Its taxonomic status was determined by using polyphasic approach. It is a Gram-positive, aerobic, non-motile, weakly acid-fast strain. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain VRC07ᵀis a member of the genus Nocardia. The highest levels of 16S rRNA gene sequence similarity was found between the strains Nocardia niwae W9241ᵀ(99.6 %), Nocardia amikacinitolerans W9988ᵀ(99.3 %) and Nocardia arthritidis IFM 10035ᵀ(98.9 %); similarity to other type strains of the genus Nocardia was below 98.7 %. The organism had chemical and morphological features consistent with its classification in the genus Nocardia such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan. Arabinose and galactose as the diagnostic sugars. Diagnostic polar lipids were phosphatidylinositol, diphosphatidylglycerol, and phosphatidylglycerol. The predominant menaquinone was MK-8(H₄, ω₋cycₗ). The major fatty acids were C₁₆:₀, C₁₈:₀, C₁₈:₁ w₉c, C₁₈:₀ ₁₀₋ₘₑₜₕyₗ TBSAand sum in feature 3 (16:1 w7c/16:1 w6c). The G+C content of the genomic DNA was 68.5 mol%. The DNA–DNA relatedness data, together with phenotypic differences clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate represents a novel species, for which the name Nocardia bhagyanesis sp. nov., is proposed. The type strain is VRC07ᵀ(=KCTC 29209ᵀ = MTCC 11725ᵀ = ATCC BAA-2548).
Agid:
621428