Jump to Main Content
Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors
- Cao, Guangli, Ximenes, Eduardo, Nichols, Nancy N., Frazer, Sarah E., Kim, Daehwan, Cotta, Michael A., Ladisch, Michael
- Bioresource technology 2015 v.190 pp. 412
- Coniochaeta ligniaria, cellulose, corn stover, enzymatic hydrolysis, enzyme inhibition, fermentation, fungi, glucose, glycosidases, hydrolysates, hydrolysis, phenolic compounds, xylooligosaccharides
- A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed.