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Determination of gamma irradiation effects on polycyclic aromatic hydrocarbons content in wheat brans and endosperms using HPLC\FLT analysis

Khalil, Ayman, Al-Daoude, Antonious
Journal für Verbraucherschutz und Lebensmittelsicherheit 2018 v.13 no.4 pp. 407-412
endosperm, fluorescence, gamma radiation, high performance liquid chromatography, polycyclic aromatic hydrocarbons, soil, tissues, traceability, wheat, wheat bran
An HPLC system equipped with a fluorescent and UV detection was deployed to determine the concentrations of 16 different polycyclic aromatic hydrocarbons (PAHs) in bran and endosperm of wheat grains before and after gamma irradiation treatments. Wheat grains were subjected to 0, 5, 10 and 15 kGy doses of gamma irradiation and concentrations of PAHs extracted from control and treated tissues were determined. Results demonstrate that accumulation of PAHs was significantly higher in wheat bran (293.1 µg × kg⁻¹) than that observed in endosperms (61.4 µg × kg⁻¹). This indicates that PAHs traceability and contamination via an atmospheric exposure is likely compared with other possible sources of a soil origin for instance. The effect of gamma irradiation dose was more effective on grain endosperm with a reduction percentage of about 92% (15 kGy) for ∑16 EPA PAHs. PAHs reduction in both tissues of studied wheat grains increased along with the applied dose of gamma irradiation.