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Correlation of spatio-temporal characteristics of intestinal inflammation with IL-17 in a rat model of hypoganglionosis

Author:
Yu, Hui, Cao, Ning-Jia, Pan, Wei-Kang, Su, Li, Zhao, Yu-Ying, Tian, Dong-Hao, Xu, Wen-Yao, Gao, Ya, Zheng, Bai-Jun
Source:
Biochemical and biophysical research communications 2018 v.506 no.4 pp. 956-961
ISSN:
0006-291X
Subject:
C-reactive protein, animal models, benzalkonium chloride, blood serum, children, colon, females, inflammation, interleukin-17, mucosa, necrosis, neoplasms, rats
Abstract:
Interleukin 17 expression is increased in children with Hirschsprung disease, which is characterized by intestinal inflammation.This study designed to exploit the characteristics of intestinal inflammation and examine the correlation of interleukin 17 in this process of hypoganglionosis model established by benzalkonium chloride treatment.Colon sections from female rats were treated with benzalkonium chloride to induce hypoganglionosis or with saline alone as a sham control. C-reactive protein and tumor necrosis factor-ɑ were used as markers of inflammation. Expression of C-reactive protein, tumor necrosis factor-ɑ, and interleukin 17 was assessed in colon tissue and blood serum on days 7, 14 and 21 after treatment. The correlation between C-reactive protein, tumor necrosis factor-ɑ, and interleukin 17 expression was estimated using the Spearman's rank-correlation coefficient.C-reactive protein, tumor necrosis factor-ɑ, and interleukin 17 were strongly expressed in submucosa and mucosa layers and serum from treated animals. The expression of C-reactive protein, tumor necrosis factor-ɑ, and interleukin 17 maintained the highest level at Day 21. Only C-reactive protein and tumor necrosis factor-ɑ expression was increased in control animals and only on day 7. Spearman's rank correlation coefficient was significant in C-reactive protein, tumor necrosis factor-ɑ, and interleukin 17 at Day 7, 14 and 21.Concomitant upregulation of C-reactive protein, tumor necrosis factor-ɑ, and interleukin 17 and significant positive correlations between C-reactive protein, tumor necrosis factor-ɑ, and interleukin 17 may imply that interleukin 17 is involved in spatio-temporal inflammation induced by benzalkonium chloride.
Agid:
6216204