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MiR-375 induces ROS and apoptosis in ST cells by targeting the HIGD1A gene

Guo, Jia, Yang, Chun, Zhang, Shaoxuan, Liang, Mengdi, Qi, Jiajia, Wang, Zhenbo, Peng, Yinghua, Sun, Boxing
Gene 2019 v.685 pp. 136-142
Sertoli cells, anaerobic conditions, apoptosis, genes, swine
HIGD1A can reduce ROS and apoptosis in cells under low-glucose or hypoxic conditions, and HIGD1A is one of the target genes for miR-375, according to our previous studies. However, it is not known whether miR-375 can indirectly regulate ROS and apoptosis in porcine Sertoli cells. To answer this question, HIGD1A and miR-375 were overexpressed in porcine Sertoli cells, and ROS and apoptosis were assayed. The results showed that ROS levels and expression levels of CASPASE3 in HIGD1A-overexpressing cells were significantly lower than those in the control cells. However, ROS levels and CASPASE3 expression in miR-375-overexpressing cells were significantly higher than those in the control cells. The rate of apoptosis in HIGD1A-overexpressing cells was significantly lower than that in miR-375-overexpressing cells. Considering that the HIGD1A gene is a target of miR-375, these findings suggest that miR-375 can induce an increase in ROS levels and apoptosis by inhibiting HIGD1A in porcine ST cells.