PubAg

Main content area

Phytochemical constituents from Uncaria rhynchophylla in human carboxylesterase 2 inhibition: Kinetics and interaction mechanism merged with docking simulations

Author:
Wang, Ya-Li, Dong, Pei-Pei, Liang, Jia-Hao, Li, Ning, Sun, Cheng-Peng, Tian, Xiang-Ge, Huo, Xiao-Kui, Zhang, Bao-Jing, Ma, Xiao-Chi, Lv, Chuan-Zhu
Source:
Phytomedicine 2018 v.51 pp. 120-127
ISSN:
0944-7113
Subject:
Oriental traditional medicine, Uncaria rhynchophylla, carboxylesterase, carboxylic acids, chemical constituents of plants, computer simulation, epilepsy, fluorescent dyes, high performance liquid chromatography, humans, hypertension, inhibitory concentration 50, kinetics, moieties, nuclear magnetic resonance spectroscopy, pre-eclampsia, serine, silica gel, triterpenoids
Abstract:
Carboxylesterases (CEs) belong to the serine hydrolase family, and are in charge of hydrolyzing chemicals with carboxylic acid ester and amide functional groups via Ser-His-Glu. Uncaria rhynchophylla (Miq.) Miq. ex Havil. is a famous traditional Chinese medicine used in managing hyperpyrexia, epilepsy, preeclampsia, and hypertension in China.To discover the potential natural human carboxylesterase 2 (hCE 2) inhibitors from U. rhynchophylla.Compounds were obtained from the hooks of U. rhynchophylla by silica gel and preparative HPLC. Their structures were elucidated by using HRESIMS, 1D and 2D NMR spectra. Their inhibitory activeties and inhibition kinetics against hCE 2 were assayed by the fluorescent probe, and potential mechanisms were also investigated by molecular docking.Twenty-three compounds, including a new phenolic acid uncariarhyine A (1), eight known triterpenoids (2–9), and ten known aromatic derivatives (10, 13–16, and 19–23), were isolated from U. rhynchophylla. Compounds 1–5, 7, 9, and 15 showed significant inhibitory activities against hCE 2 with IC50 values from 4.01 ± 0.61 µM to 18.60 ± 0.21 µM, and their inhibition kinetic analysis results revealed that compounds 1, 5, 9, and 15 were non-competitive; compounds 3 and 4 were mixed-type, and compounds 2 and 7 were uncompetitive. Molecular docking studies indicated inhibition mechanisms of compounds 1–5, 7, 9, and 15 against hCE 2.Our present findings highlight potential natural hCE 2 inhibitors from U. rhynchophylla.
Agid:
6217290