U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Enhancement of xylose utilization from corn stover by a recombinant Escherichia coli strain for ethanol production

Author:
Badal C. Saha, Nasib Qureshi, Gregory J. Kennedy, Michael A. Cotta
Source:
Bioresource technology 2015 v.190 pp. 182-188
ISSN:
0960-8524
Subject:
Escherichia coli, arabinose, bacteria, carbohydrate metabolism, corn stover, ethanol, ethanol production, fermentation, glucose, hydrolysates, inoculum, lignocellulose, pentoses, saccharification, xylose
Abstract:
Effects of substrate-selective inoculum prepared by growing on glucose, xylose, arabinose, GXA (glucose, xylose, arabinose, 1:1:1) and corn stover hydrolyzate (dilute acid pretreated and enzymatically hydrolyzed, CSH) on ethanol production from CSH by a mixed sugar utilizing recombinant Escherichia coli (strain FBR5) were investigated. The initial ethanol productivity was faster for the seed grown on xylose followed by GXA, CSH, glucose and arabinose. Arabinose grown seed took the longest time to complete the fermentation. Delayed saccharifying enzyme addition in simultaneous saccharification and fermentation of dilute acid pretreated CS by the recombinant E. coli strain FBR5 allowed the fermentation to finish in a shorter time than adding the enzyme simultaneously with xylose grown inoculum. Use of substrate selective inoculum and fermenting pentose sugars first under glucose limited condition helped to alleviate the catabolite repression of the recombinant bacterium on ethanol production from lignocellulosic hydrolyzate.
Agid:
62174
Handle:
10113/62174