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Enhancement of xylose utilization from corn stover by a recombinant Escherichia coli strain for ethanol production

Badal C. Saha, Nasib Qureshi, Gregory J. Kennedy, Michael A. Cotta
Bioresource technology 2015 v.190 pp. 182-188
Escherichia coli, arabinose, bacteria, carbohydrate metabolism, corn stover, ethanol, ethanol production, fermentation, glucose, hydrolysates, inoculum, lignocellulose, pentoses, saccharification, xylose
Effects of substrate-selective inoculum prepared by growing on glucose, xylose, arabinose, GXA (glucose, xylose, arabinose, 1:1:1) and corn stover hydrolyzate (dilute acid pretreated and enzymatically hydrolyzed, CSH) on ethanol production from CSH by a mixed sugar utilizing recombinant Escherichia coli (strain FBR5) were investigated. The initial ethanol productivity was faster for the seed grown on xylose followed by GXA, CSH, glucose and arabinose. Arabinose grown seed took the longest time to complete the fermentation. Delayed saccharifying enzyme addition in simultaneous saccharification and fermentation of dilute acid pretreated CS by the recombinant E. coli strain FBR5 allowed the fermentation to finish in a shorter time than adding the enzyme simultaneously with xylose grown inoculum. Use of substrate selective inoculum and fermenting pentose sugars first under glucose limited condition helped to alleviate the catabolite repression of the recombinant bacterium on ethanol production from lignocellulosic hydrolyzate.