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Characterization of a novel polysaccharide from the leaves of Moringa oleifera and its immunostimulatory activity

Dong, Zhuping, Li, Chao, Huang, Qiang, Zhang, Bin, Fu, Xiong, Liu, Rui Hai
Journal of functional foods 2018 v.49 pp. 391-400
Moringa oleifera, arabinose, chromatography, functional foods, galactose, glucose, immunomodulation, immunostimulants, inducible nitric oxide synthase, interleukin-6, leaves, macrophages, messenger RNA, molecular weight, nitric oxide, polysaccharides, secretion, tumor necrosis factor-alpha
A novel polysaccharide (MOP-2) was extracted and purified from the leaves of Moringa oleifera by hot water extraction and chromatographic purification. Structural characterization revealed that MOP-2 had an average molecular weight of 155.35 kDa and consisted of arabinose, glucose and galactose with molar percentages of 35.8, 6.67, and 57.53%, respectively. The backbone chain of MOP-2 was proven to be →3,6)-β-D-Galp(1→ and →6)-β-D-Galp-(1→, and the side chains contained →5)-α-L-Araf-(1→, α-L-Araf-(1→, and α-D-Glcp-(1→. In vitro immunomodulatory assay revealed that MOP-2 could significantly enhance the proliferation and pinocytic capacity of the RAW264.7 macrophage cells. Furthermore, MOP-2 promoted the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) by activating mRNA expressions of iNOS, IL-6 and TNF-α. These findings suggest that MOP-2 can be developed as a novel natural immunoregulatory agent in functional foods.