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One-Step Photochemical Generation of Biofunctionalized Hydrogel Particles via Two-Phase Flow
- Schönberg, Jan-Niklas, Zinggeler, Marc, Fosso, Patrick, Brandstetter, Thomas, Rühe, Jürgen
- ACS applied materials & interfaces 2018 v.10 no.46 pp. 39411-39416
- DNA, chemical reactions, crosslinking, diagnostic techniques, hydrogels, microfluidic technology, photochemistry, polymers, proteins
- Biofunctional hydrogel particles have become increasingly popular in medical diagnostics; however, their generation is time-consuming and typically requires several process steps. We report on a new method for the simple, fast, and reproducible one-step generation of monodisperse hydrogel particles equipped with biofunctional molecules such as proteins or DNA. Key to the approach is the simultaneous photo cross-linking of the polymer chains and covalent binding of proteins or DNA through a C,H insertion reaction inside aqueous plug compartments that are produced via microfluidics. The strong performance in biological binding assays of the functionalized particles is demonstrated.