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Identification and characterisation of Dof transcription factors in the cucumber genome

Chang-long Wen, Qing Cheng, Liqun Zhao, Aijun Mao, Jingjing Yang, Shuancang Yu, Yiqun Weng, Yong Xu
Scientific reports 2016 v.6 no.23072 pp. -
transcription factors, plant growth, biotic stress, Watermelon mosaic virus, genetic resistance, cucumbers, downy mildew, foliar diseases, microRNA, transcription (genetics), genes, abiotic stress, tissues, pathogens, defense mechanisms
Cucumber is susceptible to many foliage diseases. Although candidate genes for resistances to several pathogens in cucumber have been reported, the underlying defence mechanisms remain unclear. The Dof (DNA-binding with one finger) proteins are a group of plant-specific transcription factors that have been shown to play significant roles in plant growth, development, and responses to biotic and abiotic stresses. Dof genes can promote large-scale expression of defence-related genes at whole genome level. The gene families have been identified and characterized in several plant species, but not in cucumber. In the present study, we identified 36 CsDof gene members from the cucumber draft genome which could be classified into eight groups. The proportions of the CsDof family genes, duplication events, chromosomal locations, cis-elements and miRNA target sites were comprehensively investigated. Consequently, we analysed the expression patterns of CsDof genes in specific tissues and their response to two biotic stresses (watermelon mosaic virus and downy mildew). These results indicated that CsDof may be involved in resistance to biotic stresses in cucumber.