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Extraction, Composition and Functional Properties of Pennycress (Thlaspi arvense L.) Press Cake Protein

Mila P. Hojilla-Evangelista, Gordon W. Selling, Mark A. Berhow, Roque L. Evangelista
journal of the American Oil Chemists' Society 2015 v.92 no.6 pp. 905-914
Thlaspi arvense, acid deposition, crude protein, denaturation, emulsifying properties, emulsions, foams, heat tolerance, pH, protein isolates, sodium chloride, solubility
This study compared two methods for extracting the protein in pennycress (Thlaspi arvense L.) press cake and determined the composition and functional properties of the protein products. Proteins in pennycress press cake were extracted by using the conventional alkali-solubilization–acid-precipitation (AP) method or saline-based (SE) procedure (0.1 M NaCl at 50 °C). The extraction method has a major influence on the purity and functional properties of press cake protein products. AP had a lower protein yield (23 %) but much higher purity (90 % crude protein) compared with SE (45 % yield, 67 % crude protein). AP protein isolate had high foam capacity (120 ml), high foam stability (96 % foam volume retention) and high emulsion stability (24–35 min), and it was resistant to heat denaturation (3 % loss of solubility at pH 2 and pH 10). On the other hand, SE protein concentrate showed remarkably high solubility (>76 %) between pH 2 and 10 and exceptional emulsifying activity (226–412 m²/g protein), but was more susceptible to heat denaturation at pH 7 and pH 10 (65–78 % loss of solubility). These results strongly demonstrate that higher purity pennycress press cake protein can be produced by either saline extraction or acid precipitation and have functional properties that are desirable for non-food uses.