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A culture method for darkling beetles, Blapstinus spp. (Coleoptera: Tenebrionidae)

Author:
Zilkowski, Bruce W., Cosse, Allard A.
Source:
Journal of economic entomology 2015 v.108 no.3 pp. 1010-1013
ISSN:
0022-0493
Subject:
Alphitobius diaperinus, Cucurbitaceae, adults, apples, crops, diet, feeds, in vitro culture, insect pests, insect rearing, laboratory experimentation, pupae, sex determination analysis, zucchini, California
Abstract:
Darkling beetles, Blapstinus spp., have become a serious pest of Cucurbitaceae crops, especially in California. A culture method was sought to provide large numbers (> 500) of adult beetles of known age and sex that could be used for laboratory testing when needed. A method previously developed for Alphitobius diaperinus (Panzer) using a diet of ground chick feed, with apple slices as a moisture source, was modified for use with Blapstinus spp. and then compared to the same method substituting zucchini as the moisture source. Rearing boxes set-up with apple slices produced significantly more pupae and adults then the boxes containing zucchini slices. However, using either zucchini or apples as a moisture source, yielded over the target of 500 adults per rearing box. A previous method designed to sex A. diaperinus based on the presence (') or absence (') of second valvifers in the pupal stage also proved effective for sexing the Blapstinus spp.
Agid:
62245
Handle:
10113/62245