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Identification of Two Tobacco rattle virus Variants Associated with Line Pattern Disease of Bleeding Heart in Ohio
- Fisher, John R.
- Plant health progress 2013 v.14 no.1
- Arabis, Impatiens, Lamprocapnos spectabilis, Potyvirus, Tobacco rattle virus, alfalfa, complementary DNA, cucumbers, double-stranded RNA, enzyme-linked immunosorbent assay, host range, oligodeoxyribonucleotides, open reading frames, ornamental plants, tobacco, tomatoes, virion, weeds, Ohio
- Tobacco rattle virus (TRV) is a member of the Tobravirus genus and has a broad host range including weeds, vegetables, and ornamentals. Several bleeding heart (Lamprocapnos spectabilis) plants showing a yellow line pattern symptom were negative for the Potyvirus group, Alfalfa mosaic, Arabis mosaic, Cucumber mosaic, Impatiens necrotic spot, Tobacco mosaic, Tobacco ringspot, Tomato ringspot, and Tomato spotted wilt viruses by ELISA. cDNAs synthesized from dsRNA template and immunocaptured virions from symptomatic and asymptomatic bleeding heart amplified clear products with Tobravirus group and TRV specific primers targeted at regions in the 194 kDa open reading frame 1 on RNA 1. Cloned amplicon sequences demonstrated two distinct populations of sequences for both primer pairs and the two populations correlated with symptoms, or lack thereof, on bleeding heart. These results represent the first confirmed report of TRV associated with line pattern disease on bleeding heart in Ohio.