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Metabolomics-Driven Discovery of Meroterpenoids from a Mussel-Derived Penicillium ubiquetum

Hoang, Thi Phuong Thuy, Roullier, Catherine, Boumard, Marie-Claude, Robiou du Pont, Thibaut, Nazih, Hassan, Gallard, Jean-François, Pouchus, Yves François, Beniddir, Mehdi A., Grovel, Olivier
Journal of natural products 2018 v.81 no.11 pp. 2501-2511
Mytilus edulis, Penicillium, agar, biochemical pathways, cluster analysis, estuaries, metabolome, nuclear magnetic resonance spectroscopy, seawater, secondary metabolites, spectral analysis, tandem mass spectrometry, yeast extract, France
Penicillium ubiquetum MMS330 isolated from the blue mussel Mytilus edulis collected on the Loire estuary in France was here investigated. As very few secondary metabolites have been documented for this species, its metabolome was studied following the OSMAC approach to enhance as many biosynthetic pathways as possible. Interestingly, HPLC-HRMS based hierarchical clustering analysis together with MS/MS molecular networking highlighted the selective overproduction of some structurally related compounds when the culture was performed on seawater CYA (Czapek Yeast extract Agar) medium. Mass-guided purification from large scale cultivation on this medium led to the isolation of nine meroterpenoids including two new analogues, 22-deoxyminiolutelide A (1) and 4-hydroxy-22-deoxyminiolutelide B (2), together with seven known compounds (3–9). The structures of 1 and 2 were elucidated on the basis of HR-ESIMS and NMR spectroscopic data analysis. Furthermore, NMR signals of 22-deoxyminiolutelide B (3) were reassigned.