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Cardioprotective effects of physical exercise on redox biology in mice exposed to hand-rolled cornhusk cigarette smoke

Camera, Fernanda Dal’Maso, Pozzi, Bruna Gianatassio, Paganini, Carla de Souza, Sorato, Helen Rebelo, Tavares, Fernanda, Pereira, Bárbara da Costa, Pedroso, Giulia S., Roman, Silvane Souza, Silveira, Paulo Cesar Lock, Nesi, Renata Tiscoski, Pinho, Ricardo Aurino
Archives of biochemistry and biophysics 2019 v.661 pp. 50-55
air, cardioprotective effect, chronic exposure, cigarettes, corn husks, exercise, glutathione, glutathione peroxidase, histology, image analysis, laboratory animals, males, mice, myocardium, nitrites, oxidative stress, smoke, tissue repair
The present study sought to evaluate the effects of physical training on histological parameters and oxidative stress in the myocardium of mice chronically exposed to hand-rolled cornhusk cigarette (HRCC) smoke. Male Swiss mice (60 days old, 30–35 g) were either exposed to ambient air or passively exposed to the smoke of 12 cigarettes daily over 3 sessions (4 cigarettes per session) for 60 consecutive days with or without physical training for 8 weeks. Forty-eight hours after the last training session, the heart was surgically removed for histological analysis and measurement of oxidative stress parameters. Histological imaging revealed cell disruption, with poorly defined nuclei, in the mice exposed to HRCC smoke, but not in the control group. However, mice exposed to HRCC smoke with physical training displayed signs of tissue repair and improved tissue integrity. Biochemical analysis revealed decreased production of superoxide, 2′,7′-dichlorofluorescein (DCF), and nitrite, as well as decreased protein carbonylation, in the physical training groups, likely due to the exercise-induced increase in glutathione peroxidase (GPX) activity and glutathione (GSH) content. Taken together, our results suggest that physical exercise exerts cardioprotective effects by modulating the redox responses in animals exposed to HRCC smoke.