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Introducing nano-scale quantitative polymerase chain reaction

Author:
Dyrstad, Sissel E., Tusubira, Deusdedit, Knappskog, Stian, Tronstad, Karl J., Røsland, Gro V.
Source:
Biochemical and biophysical research communications 2018 v.506 no.4 pp. 923-926
ISSN:
0006-291X
Subject:
cost effectiveness, data quality, quantitative polymerase chain reaction
Abstract:
We show that the quantitative PCR reaction volume can be reduced significantly compared to the standard procedures, without compromising data quality. By analyzing dilution series (100–10−7) we found that measurement in 1 μl reaction volume indeed gave valid results comparable to 10 μL, when using a routine pipetting robot. This may enable a significant cost reduction through cutback of both biological material as well as chemical reagents.
Agid:
6225373