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MiRNA-224-5p inhibits autophagy in breast cancer cells via targeting Smad4

Cheng, You, Li, Zhaoyun, Xie, Jiaogui, Wang, Pan, Zhu, Jie, Li, Yueguo, Wang, Yichao
Biochemical and biophysical research communications 2018 v.506 no.4 pp. 793-798
Western blotting, autophagy, breast neoplasms, breasts, carcinogenesis, carcinoma, gene expression, messenger RNA, metastasis, microRNA, neoplasm cells, quantitative polymerase chain reaction, staining, therapeutics
Autophagy is known as a protective intracellular procedure, which can be regulated by several factors. MiRNA has been suggested as a potential element to mediate autophagy pathway in carcinomas. Our study was aim to investigate the role of autophagy in breast cancer cells and identify the involved molecular mechanismThe expression of LC3I/II, SQSTM1 and Smad4 were detected by western blot. The mRNA level were quantified by real-time PCR. MDC staining was used to directly visualize autophagosome formation. Target Scan 7.2 was used to predict biological targets of miR-224-5pMiR-224 -5p expression was upregulated in metastatic breast cancer and non-metastatic breast cancer cells compare with control. Moreover, miR-224-5p inhibition enhanced cellular autophagy levels in breast cancer cells. MiR-224-5p could suppress Smad4 expression in MDA-MB-231 cells, which indicated that Smad4 was identified as a target of miR-224-5p in breast cancer cells with high metastatic potentialOur study revealed that miR-224-5p inhibited autophagy by targeting Smad4 in MDA-MB-231 cells. The results indicated that miR-224-5p/Smad4 regulating autophagy might be a novel regulatory network contributing to metastasis of breast cancer. MiR-224-5p and Smad4 is involved in breast tumorigenesis, which is possibly a novel target for breast cancer therapy.