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Down-regulation of arginine decarboxylase gene-expression results in reactive oxygen species accumulation in Arabidopsis

Ana Isabel, Chávez-Martínez, Francisco Ignacio, Jasso-Robles, Margarita, Rodríguez-Kessler, Gill, Sarvajeet S., Alicia, Becerra-Flora, Juan Francisco, Jiménez-Bremont
Biochemical and biophysical research communications 2018 v.506 no.4 pp. 1071-1077
Arabidopsis, NAD(P)H oxidase (H2O2-forming), NADP (coenzyme), arginine decarboxylase, catalase, enzyme activity, gene expression, hydrogen peroxide, metabolism, polyamines, seedlings, superoxide anion
Arabidopsis amiR:ADC-L2 is a non-lethal line with several developmental defects, it is characterized by a drastic reduction in free polyamine content. Herein, we found that catalase application had growth-promoting effects in amiR:ADC-L2 and parental Ws seedlings. Differences in ROS content between amiR:ADC-L2 and Ws seedlings were detected. Increased H2O2 levels were found in the amiR:ADC-L2, as well as low AtCAT2 gene expression and reduced catalase activity. Estimation of polyamine oxidase activity in amiR:ADC-L2 line indicated that the over-accumulation of H2O2 is independent of polyamine catabolism. However, increments in NADPH oxidase activity and O2•− content could be associated to the higher H2O2 levels in the amiR:ADC-L2 line. Our data suggest that low polyamine levels in Arabidopsis seedlings are responsible for the accumulation of ROS, by altering the activities of enzymes involved in ROS production and detoxification.