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Cdh1 degradation is mediated by APC/C–Cdh1 and SCF–Cdc4 in budding yeast

Author:
Nagai, Masayoshi, Shibata, Atsuko, Ushimaru, Takashi
Source:
Biochemical and biophysical research communications 2018 v.506 no.4 pp. 932-938
ISSN:
0006-291X
Subject:
Saccharomyces cerevisiae, enzymes, humans, interphase, mitosis, neoplasm cells, neoplasms, phosphorylation, protein content, yeasts
Abstract:
Cdh1, a substrate-recognition subunit of anaphase-promoting complex/cyclosome (APC/C), is a tumor suppressor, and it is downregulated in various tumor cells in humans. APC/C–Cdh1 is activated from late M phase to G1 phase by antagonizing Cdk1-mediated inhibitory phosphorylation. However, how Cdh1 protein levels are properly regulated is ill-defined. Here we show that Cdh1 is degraded via APC/C–Cdh1 and Skp1–Cullin1–F-box (SCF)–Cdc4 in the budding yeast Saccharomyces cerevisiae. Cdh1 degradation was promoted by forced localization of Cdh1 into the nucleus, where APC/C and SCF are present. Cdk1 promoted APC/C–Cdh1-mediated Cdh1 degradation, whereas polo kinase Cdc5 elicited SCF–Cdc4-mediated degradation. Thus, Cdh1 degradation is controlled via multiple pathways.
Agid:
6225432